Molecular epidemiology of rabies virus isolated of herbivores from Brazilian Amazon

Brazilian Journal of Veterinary Research and Animal Science

Lucila Pereira da Silva¹  Ofir de Sales Ramos¹  Sheila Oliveira de Souza Silva²  Paulo Eduardo Brandão²  Leonardo José Richtzenhain²  Haila Chagas Peixoto²  Andrea Isabel Estévez Garcia² 

[1] Laboratório Nacional Agropecuário, Belém, PA;Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP;

关键词: herbivorous;    nucleoprotein;    glycoprotein;    phylogeny;    rabies;   

DOI  :  10.11606/issn.1678-4456.v51i2p122-130

来源: DOAJ

【 摘 要 】

Rabies virus samples (n = 17) isolated from bovines (n = 11), equines (n = 4) and buffalo (n = 2) from Pará State (n = 7), Tocantins (n = 6) and Rondônia (n = 4) were submitted to RT-PCR in order to obtain partial sequences of nucleoprotein (N) and glycoprotein gene (G). Nucleotide sequences were analyzed using Neighbor-Joining model, Kimura 2-parameters evolutionary model. All the 17 samples analyzed were related to cluster A, lineage associated with the hematophagous bat Desmodus rotundus. The phylogenetic analysis based on the N and G genes, suggests the presence of five sub-lineages (A1-A5), while G gene showed seven sub-lineages (A1-A7). In both phylogenies, sublineages A1 to A3 exhibit a similar composition and geographic distribution. Diverse composition of remaining groups of N and G gene is attributable to different sequences used in the alignments for each genomic region. Glycoprotein amino acid sequence showed molecular markers in sub-lineages A2, A3, A4 and A7. This information provides a better comprehension of molecular epidemiology of rabies, starting with the knowledge of viral lineages circulating in the Brazilian Amazon.

【 授权许可】

Unknown