Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector

Virology Journal

Xi Bao¹  Bihua Deng¹  Yiwei Wang¹  Jibo Hou¹  Yu Lu¹  Hai Xu¹  Yue Xu¹ 

[1] Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Science;

关键词: Bacteriophage T7;    Targeting delivery;    Eukaryotic expression;    Vaccine;   

DOI  :  10.1186/s12985-018-0955-1

来源: DOAJ

【 摘 要 】

Abstract Background DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide), no similar study has been conducted using T7 phage. Methods In this study, we engineeredT7 phage as a DNA targeting delivery vector to facilitate cellular internalization. We constructed recombinant T7 phages that displayed Tat peptide on their surface and carried eukaryotic expression box (EEB) as a part of their genomes (T7-EEB-Tat). Results We demonstrated that T7 phage harboring foreign gene insertion had packaged into infective progeny phage particles. Moreover, when mammalian cells that were briefly exposed to T7-EEB-Tat, expressed a significant higher level of the marker gene with the control cells infected with the wide type phage without displaying Tat peptides. Conclusion These data suggested that the potential of T7 phage as an effective delivery vector for DNA vaccine transfer.

【 授权许可】

Unknown