Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

Biosensors

Kejing Huang¹  Hui Jiang²  Jinfeng Quan²  Xuemei Wang²  Jialei Zhang²  Yihan Wang² 

[1] School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China;State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

关键词: MCF-7 cells;    electrochemistry;    2-D materials;    signal amplification;    DNA assembly;   

DOI  :  10.3390/bios11100378

来源: DOAJ

【 摘 要 】

Arch-cruciform DNA are self-assembled on AuNPs/VS₂ scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H₂O₂ reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10~1 × 10⁵ cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

【 授权许可】

Unknown