Fluorescent Signaling of Molecularly Imprinted Nanogels Prepared via Postimprinting Modifications for Specific Protein Detection

Advanced NanoBiomed Research

Yuji Nakamachi¹  Ryohei Sasaki²  Eri Takano³  Katsuki Tsutsumi³  Yukiya Kitayama³  Toshifumi Takeuchi³  Hirobumi Sunayama³ 

[1] Department of Clinical Laboratory Kobe University Hospital 7-5-2 Kusunoki-cho Chuo-ku Kobe 650-0017 Japan;Division of Radiation Oncology Kobe University Hospital 7-5-2 Kusunoki-cho Chuo-ku Kobe 650-0017 Japan;Graduate School of Engineering Kobe University 1-1, Rokkodai-cho Nada-ku Kobe 657-8501 Japan;

关键词: biosensing;    fluorescent sensing;    molecular imprinting;    polymer nanogels;    postimprinting modifications;   

DOI  :  10.1002/anbr.202000079

来源: DOAJ

【 摘 要 】

Previous reports have stated the advantages of postimprinting modification (PIM) to develop multifunctional molecularly imprinted polymers (MIPs). However, existing technologies have only been applied on bulk‐ and film‐based MIPs. Herein, the fluorescent signaling of nanogels (NGs) for protein detection is demonstrated. The NGs are prepared by molecular imprinting and PIM. A bivalent functional monomer, 4‐[2‐(N‐methacrylamido)ethylaminomethyl] benzoic acid (FM1), comprising a benzoic acid moiety for interaction with proteins and secondary amine group for incorporating fluorescent reporter molecules is used. Human serum albumin (HSA), a marker protein for human health, serves as the model protein. HSA‐imprinted NGs (MIP‐NGs) are prepared by emulsifier‐free precipitation polymerization of FM1, N‐isopropylacrylamide, 2‐methacryloxyethyl phosphorylcholine, and N,N′‐methylenebis acrylamide in the presence of HSA. After the removal of HSA, a fluorescent dye (ATTO 647 N) is treated with amino groups on FM1 residue as the PIM; this yields fluorescent‐signaling MIP‐NGs (FL‐MIP‐NGs) of ≈20 nm in diameter. The FL‐MIP‐NG‐based sensing system detects HSA in a real sample by selectively reading out the HSA‐binding events as high‐affinity fluorescence changes (Kd = 2.5 × 10−9 M). The proposed MIP‐NGs functionalized via PIMs serve as advanced functional materials for sensing biorelated compounds.

【 授权许可】

Unknown