A Caenorhabditis elegans model for ether lipid biosynthesis and function[S]

Journal of Lipid Research

John Browse¹  Pablo Tarazona²  Jennifer L. Watts²  Ivo Feussner³  Trisha J. Brock⁴  Xun Shi⁴ 

[1] Institute of Biological Chemistry, Washington State University, Pullman, WA;Department for Plant Biochemistry, Albrecht-von-Haller Institute for Plant Sciences, Georg-August-University, Gottingen, Germany;Institute of Biological Chemistry, Washington State University, Pullman, WA;School of Molecular Biosciences Washington State University, Pullman, WA;

关键词: plasmalogens;    lipidomics;    phospholipids/phosphatidylethanolamine;    fatty acid/desaturases;    peroxisomes;    C. elegans;   

DOI  :  

来源: DOAJ

【 摘 要 】

Ether lipids are widespread in nature, and they are structurally and functionally important components of membranes. The roundworm, Caenorhabditis elegans, synthesizes numerous lipid species containing alkyl and alkenyl ether bonds. We isolated C. elegans strains carrying loss-of-function mutations in three genes encoding the proteins required for the initial three steps in the ether lipid biosynthetic pathway, FARD-1/FAR1, ACL-7/GNPAT, and ADS-1/AGPS. Analysis of the mutant strains show that they lack ether lipids, but possess the ability to alter their lipid composition in response to lack of ether lipids. We found that increases in de novo fatty acid synthesis and reduction of stearoyl- and palmitoyl-CoA desaturase activity, processes that are at least partially regulated transcriptionally, mediate the altered lipid composition in ether lipid-deficient mutants. Phenotypic analysis demonstrated the importance of ether lipids for optimal fertility, lifespan, survival at cold temperatures, and resistance to oxidative stress.Caenorhabditis

【 授权许可】

Unknown