Proteomic Analysis of Methanonatronarchaeum thermophilum AMET1, a Representative of a Putative New Class of Euryarchaeota, “Methanonatronarchaeia”

Genes

Manuel Ferrer¹  Rafael Bargiela¹  Yuri I. Wolf²  Kira S. Makarova²  Eugene V. Koonin²  Sergio Ciordia³  María C. Mena³  Dimitry Y. Sorokin⁴ 

[1] Institute of Catalysis, CSIC, 28049 Madrid, Spain;National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA;Proteomics Facility, Centro Nacional de Biotecnología, CSIC, 28049 Madrid, Spain;Winogradsky Institute of Microbiology, Research Centre for Biotechnology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117312 Moscow, Russia;

关键词: proteomics;    methanogenesis;    halophiles;    Methanonatronarchaeia;    genomics;   

DOI  :  10.3390/genes9020028

来源: DOAJ

【 摘 要 】

The recently discovered Methanonatronarchaeia are extremely halophilic and moderately thermophilic methyl-reducing methanogens representing a novel class-level lineage in the phylum Euryarchaeota related to the class Halobacteria. Here we present a detailed analysis of 1D-nano liquid chromatography–electrospray ionization tandem mass spectrometry data obtained for “Methanonatronarchaeum thermophilum” AMET1 grown in different physiological conditions, including variation of the growth temperature and substrates. Analysis of these data allows us to refine the current understanding of the key biosynthetic pathways of this triple extremophilic methanogenic euryarchaeon and identify proteins that are likely to be involved in its response to growth condition changes.

【 授权许可】

Unknown