Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age

International Journal of Molecular Sciences

Jone Zuazo-Ibarra¹  María Domercq¹  Alejandro Montilla¹  Carlos Matute¹  Estibaliz Capetillo-Zarate¹  Mikel Sánchez²  Maider Beitia²  Diego Delgado²  Pello Sánchez²  Ane Garate²  AneMiren Bilbao³  Amaia Isasti⁴  Joan Sallés⁴  MaiderLópez De Jesús⁴  Imanol González-Burguera⁵  GontzalGarcía del Caño⁵ 

[1] Achucarro Basque Center for Neuroscience, CIBERNED and Departamento de Neurociencias, Universidad del País Vasco (UPV/EHU), 48940 Leioa, Spain;Advanced Biological Therapy Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain;Arthroscopic Surgery Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain;Bioaraba, Neurofarmacología Celular y Molecular, 01008 Vitoria-Gasteiz, Spain;Department of Neurosciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01008 Vitoria-Gasteiz, Spain;

关键词: platelet-rich plasma;    growth factors;    aging;    neurons;    microglia;    central nervous system;   

DOI  :  10.3390/ijms22041725

来源: DOAJ

【 摘 要 】

Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor’s health status and age. This work aims to prove the effect of PRP on cellular models of the CNS, considering the differences between PRP from young and elderly donors. Two different PRP pools were prepared from donors 65‒85 and 20‒25 years old. The cellular and molecular composition of both PRPs were analyzed. Subsequently, the cellular response was evaluated in CNS in vitro models, studying proliferation, neurogenesis, synaptogenesis, and inflammation. While no differences in the cellular composition of PRPs were found, the molecular composition of the Young PRP showed lower levels of inflammatory molecules such as CCL-11, as well as the presence of other factors not found in Aged PRP (GDF-11). Although both PRPs had effects in terms of reducing neural progenitor cell apoptosis, stabilizing neuronal synapses, and decreasing inflammation in the microglia, the effect of the Young PRP was more pronounced. In conclusion, the molecular composition of the PRP, conditioned by the age of the donors, affects the magnitude of the biological response.

【 授权许可】

Unknown