Antioxidant potential in the leaves of grape varieties (Vitis vinifera L.) grown in different soil compositions

Arabian Journal of Chemistry

Muhammad Asif¹  Ihsan ul Haq²  Hussain Shah³  Abdullah Abdullah⁴  Saad Alamri⁵  Farzana Farzana⁶  Arshad Mehmood Abbasi⁷  Yaseen Khan⁷  Shujaul Mulk Khan⁷  Mohamed Hashem⁸  Shazia Sakhi⁸ 

[1] Assiut University, Faculty of Science, Botany and Microbiology Department, Assiut 71516, Egypt;Corresponding author.;Center for Plant Sciences and Biodiversity, University of Swat, Khyber Pakhtunkhwah, Pakistan;Department of Botany, Abdul Wali Khan University, Mardan, KP, Pakistan;Department of Environmental Sciences, Abdul Wali Khan University, Mardan, Pakistan;Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan;Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan;King Khalid University, College of Science, Department of Biology, Abha 61413, Saudi Arabia;

关键词: Grape (Vitis vinifera L.) antioxidants;    Phenolic contents;    Flavonoid contents;    Reducing power;    DPPH;    Grape varieties of Pakistan;   

DOI  :  

来源: DOAJ

【 摘 要 】

Grape plant (Vitis vinifera L.) is a species of the family Vitaceae. It is an economically important fruit plant abundantly grown all over the world. Vitaceae comprised of 900 species distributed across tropical, sub-tropical and partially temperate sorts of climatic zones. In the current study we aimed to evaluate the antioxidant activities of grape leaves extract from different Pakistani varieties.We have analyzed the crude methanolic extract from the leaves of Pakistani grape varieties (i.e. NARC Black, Sundar Khani, Shogran-1, Shogran-2, Haita, Sheghali, Perlette, and Sultania-C) cultivated in various soil compositions. Total phenolic contents (TPC) of the extract were determind by using Folin-ciocalteu reagent method. Total flavonoid contents (TFC) were determind by the Aluminum Chloride Calorimetric method. Antioxidant activities of the extracts of different concentration were evaluated using Phosphomolybdenum method, Reducing power and DPPH (2, 2-disphenyl-1-picryl-hydrazyl) assays. Assays performed for grape varieties showed great antioxidant potential. All varieties significantly depicted with variation in polyphenolic contents and antioxidant potential with changes in the soil conditions. The highest TPC and TFC were quantified in Shogran-1 97.58 ± 0.09 (GAE, g−1) and Sultania-C 97.47 ± 0.12 (µg QE/g DE) varieties respectively which were grown in (T6) and (T5), while lower were recorded in Sundar khani 5.16 ± 0.23 in (T6). Likewise, Shogran-1 128.26 ± 0.23 (µg AAE/g extract) variety grown in (T6) depicted highest total antioxidant capacity, NARC black 182.06 ± 0.24 (µg AAE/g extract) variety grown in (T6) resulted in maximum reducing power while DPPH free radical scavenging potential were determinded in Sultania-C 80.91 ± 0.10 (T4) equivalent µg/g. In the terms of bioassays, the promising results were showed by compost + sand + clay (T4), compost + clay + dung (T5) and sand + clay + dung (T6). Therefore, we conclude that variation in soil composition has a significant contribution in the quantity of phytochemicals and antioxidants expression. Our study further suggest that use of organic fertilizer (Compost) and loamy sort of soil enhance functioning of most of the varieties in terms of important phytochemicals.

【 授权许可】

Unknown