Preoperative Fasting and General Anaesthesia Alter the Plasma Proteome

Cancers

Ulf Gyllensten¹  Stefan Enroth¹  Karin Sundfeldt²  Karin Stålberg³  SofiaBosdotter Enroth⁴ 

[1] Department of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Box 815, Uppsala University, SE-751 08 Uppsala, Sweden;Department of Obstetrics and Gynaecology, Institute of Clinical Sciences, Sahlgrenska Academy at Gothenburg University, SE-416 85 Gothenburg, Sweden;Department of Women’s and Children’s Health, Uppsala University, SE-751 85 Uppsala, Sweden;Medical Products Agency, Box 26, SE-751 03 Uppsala, Sweden;

关键词: plasma proteome;    preoperative conditions;    ovarian cancer;    anaesthesia;    short-time fasting;   

DOI  :  10.3390/cancers12092439

来源: DOAJ

【 摘 要 】

Background: Blood plasma collected at time of surgery is an excellent source of patient material for investigations into disease aetiology and for the discovery of novel biomarkers. Previous studies on limited sets of proteins and patients have indicated that pre-operative fasting and anaesthesia can affect protein levels, but this has not been investigated on a larger scale. These effects could produce erroneous results in case-control studies if samples are not carefully matched. Methods: The proximity extension assay (PEA) was used to characterize 983 unique proteins in a total of 327 patients diagnosed with ovarian cancer and 50 age-matched healthy women. The samples were collected either at time of initial diagnosis or before surgery under general anaesthesia. Results: 421 of the investigated proteins (42.8%) showed statistically significant differences in plasma abundance levels comparing samples collected at time of diagnosis or just before surgery under anaesthesia. Conclusions: The abundance levels of the plasma proteome in samples collected before incision, i.e., after short-time fasting and under general anaesthesia differs greatly from levels in samples from awake patients. This emphasizes the need for careful matching of the pre-analytical conditions of samples collected from controls to cases at time of surgery in the discovery as well as clinical use of protein biomarkers.

【 授权许可】

Unknown