Genome-Wide Identification, Characterization, and Expression Analysis of TUBBY Gene Family in Wheat (Triticum aestivum L.) under Biotic and Abiotic Stresses

Agronomy

Shahid Hussain¹  Ahmad Zada²  Sadia Gull³  Min Zhu⁴  Xinkai Zhu⁴  Adil Altaf⁴  Rongrong Tao⁴  Yonggang Ding⁴ 

[1] College of Agriculture, Yangzhou University, Yangzhou 225009, China;College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China;Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Key Laboratory of Crop Cultivation and Physiology, Wheat Research Center, College of Agriculture, Yangzhou University, Yangzhou 225009, China;

关键词: wheat;    temperature stress;    hormonal stress;    genes;    TaTLPs;   

DOI  :  10.3390/agronomy12051121

来源: DOAJ

【 摘 要 】

The TUBBY gene family is a group of transcription factors found in animals and plants with many functions. TLP genes have a significant role in response to different abiotic stresses. However, there is limited knowledge regarding the TUBBY gene family in T. aestivum. Here we identified 40 TaTLP genes in wheat to reveal their potential function. This study found that TUBBY (TaTLP) genes are highly conserved in wheat. The GO analysis of TaTLP genes revealed their role in growth and stress responses. Promoter analysis revealed that most TaTLPs participate in hormone and abiotic stress responses. The heatmap analysis also showed that TaTLP genes showed expression under various hormonal and abiotic stress conditions. Several genes were upregulated under different hormonal and temperature stresses. The qRT-PCR analysis confirmed our hypotheses. The results clearly indicate that various TaTLP genes showed high expression under temperature stress conditions. Furthermore, the results showed that TaTLP genes are expressed in multiple tissues with different expression patterns. For the first time in wheat, we present a comprehensive TaTLP analysis. These findings provide valuable clues for future research about the role of TLPs in the abiotic stress process in plants. Overall, the research outcomes can serve as a model for improving wheat quality through genetic engineering.

【 授权许可】

Unknown