Nasal Microbiome Change During and After Exacerbation in Asthmatic Children

Frontiers in Microbiology

Pei-Chi Chen²  Miao-Hsi Hsieh²  Wen-Shuo Kuo²  Jiu-Yao Wang³  Cheng-Han Lin⁴  Tsunglin Liu⁴  Shuen-Lin Jeng⁵  Lawrence Shih-Hsin Wu⁶  Hui-Ju Tsai⁷  Yi-Lin Chen⁸  Chung-Liang Ho⁸ 

[1]Allergy and Clinical Immunology Research (ACIR) Center, National Cheng Kung University, Tainan, Taiwan

[2]Center of Allergy, Immunology, and Microbiome (AIM), China Medical University Children’s Hospital, Taichung, Taiwan

[3]Department of Allergy and Immunology, China Medical University Children’s Hospital, Taichung, Taiwan

[4]Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, Taiwan

[5]Department of Statistics, Center for Innovative Fin Tech Business Models, Institute of Data Science, National Cheng Kung University, Tainan, Taiwan

[6]Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan

[7]Institute of Population Health Sciences, National Health Research Institutes, Zhunan, Taiwan

[8]Molecular Diagnostic Laboratory, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan

关键词: childhood asthma;    acute exacerbation;    mite allergy;    nasal microbiota;    recovery phase;   

DOI  :  10.3389/fmicb.2021.833726

来源: DOAJ

【 摘 要 】

Airway and gut microbiota are important in asthma pathogenesis. Although several studies have revealed distinct microbiota in asthmatic airways at baseline compared to healthy controls, limited studies compared microbiota during acute exacerbation (AE) and in the recovery phase (RP) in the same asthmatic children. We aim to investigate association between microbiota and asthma status in children and explore their relationship with clinical features of asthma. We recruited 56 asthmatic children and investigated their nasal, throat, and stool microbiota during AE and in the RP. Totally, 320 samples were subjected to 16S rRNA sequencing. Although the microbial communities were clearly separated by body site, within each site the overall communities during AE and in the RP could not be distinguished. Most nasal microbiota were dominated by only one or two of six bacterial genera. The domination was associated with mite allergy and patient age only during AE but not in the RP. When moving into RP, the relative abundance of Staphylococcus increased while that of Moraxella decreased. Throat and stool microbiota were not associated with most of the clinical features. Interestingly, stool microbiota during AE was associated with ABO blood type and stool microbiota in the RP was associated with frequency of the subsequent exacerbations. In summary, the association between nasal microbiota and mite allergy only during AE suggests an altered local immunity and its interplay with nasal microbes. Our work provides a basis for studying microbes, and prevention or therapeutic strategy in childhood asthma, especially during AE.

【 授权许可】

Unknown