The Effect of the PVA/Chitosan/Citric Acid Ratio on the Hydrophilicity of Electrospun Nanofiber Meshes

Polymers

Ferenc Tóth¹  Zsuzsanna Czibulya¹  Csaba Hegedűs¹  István Csarnovics²  Petra Pál²  Attila Csík³  Romána Zelkó⁴ 

[1] Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, 98. Nagyerdei Blvd, H-4032 Debrecen, Hungary;Department of Experimental Physics, Faculty of Science and Technology, University of Debrecen, 18/a Bem Square, H-4002 Debrecen, Hungary;Laboratory of Materials Science, Institute for Nuclear Research (ATOMKI), 18/c Bem Square, H-4026 Debrecen, Hungary;University Pharmacy Department of Pharmacy Administration, Semmelweis University, 7–9 Hőgyes Street, H-1092 Budapest, Hungary;

关键词: electrospinning;    polyvinyl alcohol;    chitosan;    citric acid;    hydrophilicity;   

DOI  :  10.3390/polym13203557

来源: DOAJ

【 摘 要 】

In this study, scaffolds were prepared via an electrospinning method for application in oral cavities. The hydrophilicity of the fiber mesh is of paramount importance, as it promotes cell spreading; however, the most commonly used polyvinyl alcohol (PVA) and other hydrophilic fiber meshes immediately disintegrate in aqueous media. In contrast, the excessive hydrophobicity of the scaffolds already inhibits cells adhesion on the surface. Therefore, the hydrophilicity of the fiber meshes needed to be optimized. Scaffolds with different polyvinyl alcohol (PVA)/chitosan/citric acid ratios were prepared. The addition of chitosan and the heat initiated cross-linkage of the polymers via citric acid enhanced the scaffolds’ hydrophobicity. The optimization of this property could be followed by contact angle measurements, and the increased number of cross-linkages were also supported by IR spectroscopy results. The fibers’ physical parameters were monitored via low-vacuum scanning electron microscopy (SEM) and atomic force microscopy (AFM). As biocompatibility is essential for dental applications, Alamar Blue assay was used to prove that meshes do not have any negative effects on dental pulp stem cells. Our results showed that the optimization of the fiber nets was successful, as they will not disintegrate in intraoral cavities during dental applications.

【 授权许可】

Unknown