Potential Survival and Pathogenesis of a Novel Strain, Vibrio parahaemolyticus FORC_022, Isolated From a Soy Sauce Marinated Crab by Genome and Transcriptome Analyses

Frontiers in Microbiology

Sang H. Choi²  Hyunjin Yoon⁵  Kyu-Ho Lee⁹  Eun J. Na^1,10  Ju-Hoon Lee^1,10  Hyeun B. Kim^1,10  Byungho Lee^1,10  Hee G. Jeong^1,10  Sangryeol Ryu^1,10  Han Y. Chung^1,10  Bong-Soo Kim^1,10  Heebal Kim^1,10 

[1] Biological Engineering, Ajou University, Suwon, South Korea;Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, South Korea;Department of Animal Resources Science, Dankook University, Cheonan, South Korea;Department of Animal Science and Biotechnology, Seoul National University, Seoul, South Korea;;Department of Applied Chemistry &Department of Food Science and Biotechnology, Kyung Hee University, Yongin, South Korea;Department of Food Science and Technology, Chungnam National University, Daejeon, South Korea;Department of Life Science, Multidisciplinary Genome Institute, Hallym University, Chuncheon, South Korea;Department of Life Science, Sogang University, Seoul, South Korea;Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul, South Korea;

关键词: Vibrio parahaemolyticus;    whole genome sequencing;    genomic comparison;    transcriptome;    virulence factors;    crab;   

DOI  :  10.3389/fmicb.2018.01504

来源: DOAJ

【 摘 要 】

Vibrio parahaemolyticus can cause gastrointestinal illness through consumption of seafood. Despite frequent food-borne outbreaks of V. parahaemolyticus, only 19 strains have subjected to complete whole-genome analysis. In this study, a novel strain of V. parahaemolyticus, designated FORC_022 (Food-borne pathogen Omics Research Center_022), was isolated from soy sauce marinated crabs, and its genome and transcriptome were analyzed to elucidate the pathogenic mechanisms. FORC_022 did not include major virulence factors of thermostable direct hemolysin (tdh) and TDH-related hemolysin (trh). However, FORC_022 showed high cytotoxicity and had several V. parahaemolyticus islands (VPaIs) and other virulence factors, such as various secretion systems (types I, II, III, IV, and VI), in comparative genome analysis with CDC_K4557 (the most similar strain) and RIMD2210633 (genome island marker strain). FORC_022 harbored additional virulence genes, including accessory cholera enterotoxin, zona occludens toxin, and tight adhesion (tad) locus, compared with CDC_K4557. In addition, O3 serotype specific gene and the marker gene of pandemic O3:K6 serotype (toxRS) were detected in FORC_022. The expressions levels of genes involved in adherence and carbohydrate transporter were high, whereas those of genes involved in motility, arginine biosynthesis, and proline metabolism were low after exposure to crabs. Moreover, the virulence factors of the type III secretion system, tad locus, and thermolabile hemolysin were overexpressed. Therefore, the risk of foodborne-illness may be high following consumption of FORC_022 contaminated crab. These results provided molecular information regarding the survival and pathogenesis of V. parahaemolyticus FORC_022 strain in contaminated crab and may have applications in food safety.

【 授权许可】

Unknown