TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres

eLife

Pui Pik Law¹  Holger Kramer¹  Emilia Herrera-Moyano¹  Alex Montoya¹  Eleni Skourti²  Peter Faull²  Rosa Maria Porreca²  Jean-Baptiste Vannier²  Roser Gonzalez Franco² 

[1] Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, United Kingdom;Telomere Replication and Stability group, Medical Research Council - London Institute of Medical Sciences, London, United Kingdom;

关键词: telomere;    DNA damage response;    replication;    DNA recombination;    TERRA;    ALT;   

DOI  :  10.7554/eLife.49817

来源: DOAJ

【 摘 要 】

Telomeres are a significant challenge to DNA replication and are prone to replication stress and telomere fragility. The shelterin component TRF1 facilitates telomere replication but the molecular mechanism remains uncertain. By interrogating the proteomic composition of telomeres, we show that mouse telomeres lacking TRF1 undergo protein composition reorganisation associated with the recruitment of DNA damage response and chromatin remodellers. Surprisingly, mTRF1 suppresses the accumulation of promyelocytic leukemia (PML) protein, BRCA1 and the SMC5/6 complex at telomeres, which is associated with increased Homologous Recombination (HR) and TERRA transcription. We uncovered a previously unappreciated role for mTRF1 in the suppression of telomere recombination, dependent on SMC5 and also POLD3 dependent Break Induced Replication at telomeres. We propose that TRF1 facilitates S-phase telomeric DNA synthesis to prevent illegitimate mitotic DNA recombination and chromatin rearrangement.

【 授权许可】

Unknown