Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

Indonesian Journal of Chemistry

Suharjono Suharjono¹  Tri Ardyati¹  Akyunul Jannah²  Aulanni`am Aulanni`am³ 

[1] Department of Biology, Brawijaya University, Jl. Veteran 6, Malang 65144, East Java, Indonesia;Department of Chemistry, Maulana Malik Ibrahim State Islamic University Malang, Jl. Gajayana 50 Dinoyo, Malang 65144, East Java, Indonesia;Faculty of Veterinary Medicine, Brawijaya University, Jl. Puncak Dieng Dau, Malang 65151, East Java, Indonesia;

关键词: cellulase enzyme;    rice bran;    16S rDNA method;    Bacillus subtillis;    Bacillus cereus;   

DOI  :  10.22146/ijc.26783

来源: DOAJ

【 摘 要 】

Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 16S rDNA showed that BE 8 belongs to Bacillus subtilis and BE 14 in Bacillus cereus.

【 授权许可】

Unknown