Ultrasensitive Ti3C2TX MXene/Chitosan Nanocomposite-Based Amperometric Biosensor for Detection of Potential Prostate Cancer Marker in Urine Samples

Processes

Aisha Tanvir¹  Peter Kasak¹  Jan Tkac²  Stefania Hroncekova²  Tomas Bertok²  Alica Vikartovska²  Michal Hires²  Eduard Jane²  Lenka Lorencova² 

[1] Center for Advanced Materials, Qatar University, P. O. BOX 2713, Doha, Qatar;Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia;

关键词: MXene;    nanocomposite;    biosensor;    sarcosine;    sarcosine oxidase;    prostate cancer;   

DOI  :  10.3390/pr8050580

来源: DOAJ

【 摘 要 】

Two-dimensional layered nanomaterial Ti₃C₂T_X (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical measurements, SEM and AFM analysis revealed that MXene interfaced with chitosan is an excellent support for enzyme immobilisation to fabricate a sensitive biosensor exhibiting a low detection limit of 18 nM and a linear range up to 7.8 µM. The proposed biosensing method also provides a short response time of 2 s and high recovery index of 102.6% for detection of sarcosine spiked into urine sample in a clinically relevant range.

【 授权许可】

Unknown