The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells

Micromachines

Barbara Eva Maria Bachmann¹  Sarah Spitz²  Mario Rothbauer²  Peter Ertl²  Christoph Eilenberger²  Eva Kathrin Ehmoser³ 

[1] Austrian Cluster for Tissue Regeneration and Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Allgemeine Unfallversicherungsanstalt (AUVA) Research Centre, A-1200 Vienna, Austria;Institute of Applied Synthetic Chemistry, Institute of Chemical Technologies and Analytics, Faculty of Technical Chemistry, Vienna University of Technology, A-1060 Vienna, Austria;Institute of Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences Vienna, A-1190 Vienna, Austria;

关键词: synthetic biology;    microfluidics;    high throughput;    artificial cells;    protein synthesis;    cell-free synthesis;    droplets;    bottom-up;    top-down;   

DOI  :  10.3390/mi10050285

来源: DOAJ

【 摘 要 】

Synthetic biology aims to understand fundamental biological processes in more detail than possible for actual living cells. Synthetic biology can combat decomposition and build-up of artificial experimental models under precisely controlled and defined environmental and biochemical conditions. Microfluidic systems can provide the tools to improve and refine existing synthetic systems because they allow control and manipulation of liquids on a micro- and nanoscale. In addition, chip-based approaches are predisposed for synthetic biology applications since they present an opportune technological toolkit capable of fully automated high throughput and content screening under low reagent consumption. This review critically highlights the latest updates in microfluidic cell-free and cell-based protein synthesis as well as the progress on chip-based artificial cells. Even though progress is slow for microfluidic synthetic biology, microfluidic systems are valuable tools for synthetic biology and may one day help to give answers to long asked questions of fundamental cell biology and life itself.

【 授权许可】

Unknown