Population Genomics of American Mink Using Whole Genome Sequencing Data

Genes

Karim Karimi¹  Younes Miar¹  Duy Ngoc Do¹  Mehdi Sargolzaei² 

[1] Department of Animal Science and Aquaculture, Dalhousie University, Truro, NS B2N 5E3, Canada;Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada;

关键词: American mink;    linkage disequilibrium;    population structure;    whole genome sequencing;   

DOI  :  10.3390/genes12020258

来源: DOAJ

【 摘 要 】

Characterizing the genetic structure and population history can facilitate the development of genomic breeding strategies for the American mink. In this study, we used the whole genome sequences of 100 mink from the Canadian Centre for Fur Animal Research (CCFAR) at the Dalhousie Faculty of Agriculture (Truro, NS, Canada) and Millbank Fur Farm (Rockwood, ON, Canada) to investigate their population structure, genetic diversity and linkage disequilibrium (LD) patterns. Analysis of molecular variance (AMOVA) indicated that the variation among color-types was significant (p < 0.001) and accounted for 18% of the total variation. The admixture analysis revealed that assuming three ancestral populations (K = 3) provided the lowest cross-validation error (0.49). The effective population size (Ne) at five generations ago was estimated to be 99 and 50 for CCFAR and Millbank Fur Farm, respectively. The LD patterns revealed that the average r² reduced to <0.2 at genomic distances of >20 kb and >100 kb in CCFAR and Millbank Fur Farm suggesting that the density of 120,000 and 24,000 single nucleotide polymorphisms (SNP) would provide the adequate accuracy of genomic evaluation in these populations, respectively. These results indicated that accounting for admixture is critical for designing the SNP panels for genotype-phenotype association studies of American mink.

【 授权许可】

Unknown