Occurrence of extended-spectrum β-lactamase, AmpC, and carbapenemase-producing genes in gram-negative bacterial isolates from human immunodeficiency virus infected patients

Journal of Infection and Public Health

Balasubramanian Senthamilselvan¹  Chinnambedu Ravichandran Swathirajan²  Reem M. Aljowaie³  Tse-Wei Chen⁴  Sunil Suhas Solomon⁵  Narasingam Arunagirinathan⁵  Marimuthu Ragavan Rameshkumar⁵  Pachamuthu Balakrishnan⁶  Khalid S. Almaary⁷  Ramachandran Vignesh⁷ 

[1]Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, India

[2]Corresponding author at: Department of Microbiology and Biotechnology, Presidency College (Autonomous), Chennai, 600 005, Tamil Nadu, India.

[3]Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, USA

[4]Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur Royal College of Medicine Perak (RCMP UniKL), Ipoh, Malaysia

[5]Department of Microbiology and Biotechnology, Presidency College (Autonomous), Affiliated to University of Madras, Chennai, Tamil Nadu, India

[6]HIV Clinic, Y.R. Gaitonde Centre for AIDS Research and Education, Voluntary Health Services Hospital Campus, Chennai, India

[7]Infectious Diseases Laboratory, Y.R. Gaitonde Centre for AIDS Research and Education, Voluntary Health Services Hospital Campus, Chennai, India

关键词: HIV patients;    Klebsiella pneumoniae;    Extended-spectrum β-lactamases;    Multidrug-resistant;    blaCTX-M;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Progressive decline of immune response in HIV patients makes them susceptible to frequent bacterial infections. High usage of antibiotics influences the emergence of multidrug-resistant bacteria and worsens the clinical outcomes. In this study, the occurrence of drug-resistant genes in Gram-negative bacterial isolates from HIV patients in South India was analyzed. Methods: A total of 173 Gram-negative bacterial (GNB) isolates from HIV patients were screened for antibiotic susceptibility profile using the Kirby-Bauer diskdiffusion method. Positivity of drug-resistant genes was analyzed using polymerase chain reaction method. Results: In this study, 72.8% of bacterial isolates were obtained from urine specimens, and Escherichia coli (47.4%) was the predominantly isolated bacterium. Overall, 87.3% and 83.2% of GNB were resistant to 3rd generation cephalosporin antibiotics such as cefotaxime and ceftazidime, respectively, 56.6% were resistant to cephamycin (cefoxitin) and 43% to carbapenem (imipenem) antibiotics. Extended-spectrum β-lactamases (ESBL) production was noted among 79.5% of GNB isolates, followed by AmpC (57.1%) and Metallo β-lactamases (37.3%). Molecular analysis revealed that ESBL genes such as blaTEM (94.1%), blaCTX-M (89.2%), and blaSHV (24.2%) were detected at higher levels among GNB isolates. Carbapenemase-producing genes such as blaOXA-48 (20%), blaOXA-23 (2.6%), and both blaOXA-23 and blaOXA-51 like genes (2.6%) and AmpC producing genes such as blaCIT (26.7%), blaDHA (3.6%), and blaACC (1.8%) were detected at low-level. Conclusions: This study concludes that ESBL producing genes are detected at high level among gram-negative bacterial isolates from HIV patients in South India.

【 授权许可】

Unknown