Detection of Trichomoniasis in cattle in Nineveh province

Iraqi Journal of Veterinary Sciences

Sadam D. Hassan¹  Qaes T. Alobaidii²  Wasan A. Alobaidii³ 

[1] Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq;Department of Internal and preventive medicine, College of Veterinary Medicine, University of Mosul, Mosul, Iraq;Department of Microbiology,College of Veterinary medicine ,University of Mosul,Mosul,Iraq;

关键词: tritrichomonas foetus;    cow;    clinical status;    cpcr;   

DOI  :  10.33899/ijvs.2020.126790.1380

来源: DOAJ

【 摘 要 】

The current study was conducted on the detection of Tritrichomonas foetus in vaginal mucus of infected cows using conventional polymerase chain reaction technique (cPCR) in Nineveh province, Iraq. A total of 87 vaginal mucus samples were collected randomly from the vagina of Heifer cows of different ages (2-4, >4-6, >6 years old) and stages of pregnancy with different clinical status (early embryonic death, pyometra, abortion and healthy animals once) by washing cow’s vagina using artificial insemination pipette, DNA extraction of T. foetus was done from vaginal mucus samples, cPCR was attempt using TFR3 and TFR4 primers, Results indicated that 11 cows (12.6 %) were positive for T. foetus. The clinical status of cows demonstrated statistically significant (P<0.05) a higher percentage of Early Embryonic Death at (6.9%) compared to pyometra, abortion, and healthy cows. Furthermore, the percentage of T. foetusinfection was significantly (P<0.05) elevated among cows (>2-4 years old) at (8%) compared to (>4-6 years old) and (>6 years old) cows. This study concluded that T. foetusinfection was an elevated percentage of infection in cows with early embryonic death and in cows (>2-4) years old. This study is the first detected T. foetus in cattle in Nineveh province.

【 授权许可】

Unknown