Time-Dependent Response of Human Deciduous Tooth-Derived Dental Pulp Cells Treated with TheraCal LC: Functional Analysis of Gene Interactions Compared to MTA

Journal of Clinical Medicine

Young Kim¹  Jae-Hwan Kim²  OkHyung Nam³  SungChul Choi³ 

[1] Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju 61186, Korea;Department of Pediatric Dentistry, School of Dentistry, Chonnam National University, Gwangju 61186, Korea;Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, Korea;

关键词: gene expression;    mineral trioxide aggregate;    mrna-sequencing;    theracal lc;    vital pulp therapy;   

DOI  :  10.3390/jcm9020531

来源: DOAJ

【 摘 要 】

Pulp capping material should facilitate hard tissue regeneration on the injured pulp tissue. TheraCal LC (TC) was recently developed. Although TC has shown reliable clinical outcomes after direct pulp capping, there are still remaining concerns regarding its detrimental effect on pulp cells. Therefore, this study aimed to identify the gene expression of human deciduous tooth-derived dental pulp cells exposed to TC compared to mineral trioxide aggregate (MTA). The cells were cultured and exposed to TC and MTA for 24 and 72 h. Next, total RNA was isolated. QuantSeq 3′ mRNA-sequencing was used to examine differentially expressed genes (DEGs) in exposed to TC and MTA. Functional analysis of DEGs was performed using bioinformatics analysis. In gene ontology (GO) functional enrichment analysis, cells in TC for 24 h presented significantly enriched immune response (p < 0.001) and inflammatory response (p < 0.01) compared to MTA. TC showed enriched positive regulation of cell migration at 72 h (p < 0.001). In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, neuroactive ligand−receptor interaction (p = 1.19 × 10⁻⁷) and calcium signaling pathway (p = 2.96 × 10⁻⁵) were confirmed in the shared DEGs in TC. In conclusion, DEGs in TC may be involved in pathways associated with osteoclastogenesis and osteoclastic differentiation.

【 授权许可】

Unknown