Proteomic Changes in Antioxidant System in Strawberry During Ripening

Frontiers in Plant Science

Mindy Vinqvist-Tymchuk¹  Charles Forney¹  Sherry Fillmore¹  Jun Song¹  Leslie CampbellPalmer¹  Honghui Luo²  Zhaoqi Zhang² 

[1] Agriculture and Agri-Food Canada, Kentville Research and Development Centre, Kentville, NS, Canada;College of Horticulture, South China Agriculture University, Guangzhou, China;

关键词: Fragaria × ananassa;    fruit ripening;    quality;    multiple reaction monitoring;    OFFGEL;    proteomics;   

DOI  :  10.3389/fpls.2020.594156

来源: DOAJ

【 摘 要 】

To investigate the strawberry antioxidant defense system during fruit ripening, a targeted quantitative proteomic approach using multiple reaction monitoring (MRM) was developed to investigate targeted proteins in the antioxidant enzyme system in strawberry fruit. We investigated 46 proteins and isoforms with 73 identified peptides which may be involved in this antioxidant enzyme system. Among the proteins that changed during ripening, aldo/keto reductase (AKR), superoxide dismutase (SOD) and glutathione transferase (GT) increased significantly, while dehydroascorbate reductase, 2-Cys peroxiredoxin, catalase (CAT), 1-Cys peroxiredoxin and L-ascorbate peroxidase (APX) decreased significantly. These results suggest that fruit ripening of strawberry activates the enzymes of an SOD/glutathione metabolism system. The methodologies used in this study will be useful for systematically characterizing the role of antioxidant enzymes in fruit ripening of other plants.

【 授权许可】

Unknown