Evaluation in a Cytokine Storm Model In Vivo of the Safety and Efficacy of Intravenous Administration of PRS CK STORM (Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells)

Biomedicines

Alicia Algaba¹  Fernando Bermejo²  Jara Sanz³  Paula Desportes³  Alberto Anel⁴  Joaquín Marco-Brualla⁴  Juan Víctor San Martín⁵  Mario García-Gil⁶  Daniel Lapuente⁷  Belén Lapuente⁷  Pablo Fernández⁷  Sergio Gómez⁷  Gonzalo Gómez⁷  Juan Pedro Lapuente⁷  María de las Viñas Andrés⁷  Juan Carlos De Gregorio⁷  Almudena De Gregorio⁷ 

[1] Clinical Assay Department, Fuelabrada Hospital, 28942 Madrid, Spain;Digestive Department, Fuenlabrada Hospital, 28942 Madrid, Spain;GMP Facility, Peaches Biotech, 28050 Madrid, Spain;Group Immunity, Cancer and Stem Cells, Faculty of Sciences, University of Zaragoza, 50009 Zaragoza, Spain;Internal Medicine Department, Fuenlabrada Hospital, 28942 Madrid, Spain;Pharmacy Department, Fuenlabrada Hospital, 28942 Madrid, Spain;R4T Molecular and Cell Biology Research Laboratories, Fuenlabrada Hospital, 28942 Madrid, Spain;

关键词: PRS CK STORM;    cytokine storm;    mesenchymal stem cells;    M2 macrophages;    coculture;    crosstalk;   

DOI  :  10.3390/biomedicines10051094

来源: DOAJ

【 摘 要 】

Our research group has been developing a series of biological drugs produced by coculture techniques with M2-polarized macrophages with different primary tissue cells and/or mesenchymal stromal cells (MSC), generally from fat, to produce anti-inflammatory and anti-fibrotic effects, avoiding the overexpression of pro-inflammatory cytokines by the innate immune system at a given time. One of these products is the drug PRS CK STORM, a medium conditioned by allogenic M2-polarized macrophages, from coculture, with those macrophages M2 with MSC from fat, whose composition, in vitro safety, and efficacy we studied. In the present work, we publish the results obtained in terms of safety (pharmacodynamics and pharmacokinetics) and efficacy of the intravenous application of this biological drug in a murine model of cytokine storm associated with severe infectious processes, including those associated with COVID-19. The results demonstrate the safety and high efficacy of PRS CK STORM as an intravenous drug to prevent and treat the cytokine storm associated with infectious processes, including COVID-19.

【 授权许可】

Unknown