Genetic Transformation in Cryptococcus Species

Journal of Fungi

Ping Wang¹ 

[1] Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, LO 70112, USA;

关键词: Cryptococcus;    selectable gene marker;    electroporation;    biolistic transformation;    CRISPR-Cas9 technique;   

DOI  :  10.3390/jof7010056

来源: DOAJ

【 摘 要 】

Genetic transformation plays an imperative role in our understanding of the biology in unicellular yeasts and filamentous fungi, such as Saccharomyces cerevisiae, Aspergillus nidulans, Cryphonectria parasitica, and Magnaporthe oryzae. It also helps to under the virulence and drug resistance mechanisms of the pathogenic fungus Cryptococcus that causes cryptococcosis in health and immunocompromised individuals. Since the first attempt at DNA transformation in this fungus by Edman in 1992, various methods and techniques have been developed to introduce DNA into this organism and improve the efficiency of homology-mediated gene disruption. There have been many excellent summaries or reviews covering the subject. Here we highlight some of the significant achievements and additional refinements in the genetic transformation of Cryptococcus species.

【 授权许可】

Unknown