Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved

International Journal of Molecular Sciences

Shailendra Gupta¹  Julia Scheel¹  Eva García-Lopez²  José Luis García-Gimenez²  José Santiago Ibañez-Cabellos²  Salvador Mena-Mollá²  José Morales-Roselló³  Gabriela Loscalzo³  Alfredo Perales-Marín³ 

[1] Department of Systems Biology and Bioinformatics, University Rostock, 18055 Rostock, Germany;EpiDisease S.L, Parc Científic, University of Valencia, 46980 Paterna, Spain;Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain;

关键词: late-onset fetal growth restriction;    miRNA;    network analysis;    bioinformatics;    fetal health;    cerebroplacental ratio;   

DOI  :  10.3390/ijms23010293

来源: DOAJ

【 摘 要 】

In a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of miRNAs from neonatal cord blood belonging to LO-FGR fetuses, was validated by real-time PCR. In addition, functional characterization of overexpressed miRNAs was performed by analyzing overrepresented pathways, gene ontologies, and prioritization of synergistically working miRNAs. Three miRNAs: miR-25-3p, miR-185-5p and miR-132-3p, were significantly overexpressed in cord blood of LO-FGR fetuses. Pathway and gene ontology analysis revealed over-representation of certain molecular pathways associated with cardiac development and neuron death. In addition, prioritization of synergistically working miRNAs highlighted the importance of miR-185-5p and miR-25-3p in cholesterol efflux and starvation responses associated with LO-FGR phenotypes. Evaluation of miR-25-3p; miR-132-3p and miR-185-5p might serve as molecular biomarkers for the diagnosis and management of LO-FGR; improving the understanding of its influence on adult disease.

【 授权许可】

Unknown