Effect of Cold Storage on the Viable and Total Bacterial Populations in Human Milk

Nutrients

Michelle L. Trevenen¹  Donna T. Geddes²  Lisa F. Stinson² 

[1] Centre for Applied Statistics, The University of Western Australia, Perth, WA 6000, Australia;School of Molecular Sciences, The University of Western Australia, Perth, WA 6000, Australia;

关键词: human milk;    microbiome;    bacteria;    storage;    viability;    expressed breast milk;   

DOI  :  10.3390/nu14091875

来源: DOAJ

【 摘 要 】

Expression and cold storage of human milk is a common practice. Current guidelines for cold storage of expressed milk do not take into account the impact on the milk microbiome. Here, we investigated the impact of cold storage on viable bacterial populations in human milk. Freshly expressed milk samples (n = 10) were collected and analysed immediately, stored at 4 °C for four days, −20 °C for 2.25 months and 6 months, and −80 °C for 6 months. Samples were analysed using propidium monoazide (PMA; a cell viability dye) coupled with full-length 16S rRNA gene. An aliquot of each sample was additionally analysed without PMA to assess the impact of cold storage on the total DNA profile of human milk. Cold storage significantly altered the composition of both the viable microbiome and total bacterial DNA profile, with differences in the relative abundance of several OTUs observed across each storage condition. However, cold storage did not affect the richness nor diversity of the samples (PERMANOVA all p > 0.2). Storage of human milk under typical and recommended conditions results in alterations to the profile of viable bacteria, with potential implications for infant gut colonisation and infant health.

【 授权许可】

Unknown