Cytoprotective Effects of Mangiferin and Z-Ligustilide in PAH-Exposed Human Airway Epithelium in Vitro

Nutrients

Inga Stasiulaitienė¹  Dainius Martuzevičius¹  PetrasRimantas Venskutonis²  Dovilė Grauzdytė²  Ieva Kulvinskienė³  Jovilė Raudoniūtė³  Daiva Bironaitė³  Edvardas Bagdonas³  Rūta Aldonytė³ 

[1] Department of Environmental Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas LT-50264, Lithuania;Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas LT-50254, Lithuania;State research Institute Center for Innovative Medicine, Santariskiu 5, Vilnius 08406, Lithuania;

关键词: PAH;    mangiferin;    Z-ligustilide;    BEAS-2B;    cytotoxicity;    antioxidant capacity;   

DOI  :  10.3390/nu11020218

来源: DOAJ

【 摘 要 】

According to World Health Organisation (WHO) air pollution increases the risk of cardiovascular disorders, respiratory diseases, including COPD, lung cancer and acute respiratory infections, neuro-degenerative and other diseases. It is also known that various phytochemicals may mitigate such risks. This study tested if phytochemicals mangiferin (MNG) and Z-ligustilide (Z-LG) may protect PAH-exposed human lung bronchial epithelial cells (BEAS-2B). Organic PAH extract was obtained from the urban fine PM with high benzo(a)pyrene content collected in Eastern European mid-sized city during winter heating season. Cell proliferation traits and levels of intracellular oxidative stress were examined. Effect of MNG (0.5 µg/mL) alone or in combination with PAH on bronchial epithelium wound healing was evaluated. Both phytochemicals were also evaluated for their antioxidant properties in acellular system. Treatment with MNG produced strong cytoprotective effect on PAH-exposed cells (p < 0.01) while Z-LG (0.5 µg/mL) exhibited strong negative effect on cell proliferation in untreated and PAH-exposed cells (p < 0.001). MNG, being many times stronger antioxidant than Z-LG in chemical in vitro assays (p < 0.0001), was also able to decrease PAH-induced oxidative stress in the cell cultures (p < 0.05). Consequently MNG ameliorates oxidative stress, speeds up wound healing process and restores proliferation rate in PAH-exposed bronchial epithelium. Such protective effects of MNG in air pollution affected airway epithelium stimulate further research on this promising phytochemical.

【 授权许可】

Unknown