Population-based Laboratory Surveillance for AmpC β-Lactamase–producing Escherichia coli, Calgary

Emerging Infectious Diseases

关键词: Surveillance;    E. coli;    AmpC β-lactamases;    community-onset infections;    research;    Canada;   

DOI  :  10.3201/eid1303.060447

来源: DOAJ

【 摘 要 】

In the Calgary Health Region during 2000–2003, prospective, active, population-based laboratory surveillance for all cefoxitin-resistant Escherichia coli isolates was performed. Isolates were screened with an inhibitor-based disk test, and plasmid-mediated types were identified by multiplex PCR with sequencing. A total of 369 AmpC β-lactamase–producing E. coli isolates were identified; annual incidence rates were 1.7, 4.3, 11.2, and 15 per 100,000 residents for each year, respectively. AmpC β-lactamase–producing E. coli was 5× more likely to be isolated from female than male patients across all age groups except <1 year. Of these isolates, 83% were community onset, and urine was the principal site of isolation (90% of patients). PCR showed that 125 (34%) were positive for blacmy genes; sequencing identified these enzymes to be CMY-2. In this large Canadian region, AmpC β-lactamase–producing E. coli is an emerging community pathogen that commonly causes urinary tract infections in older women.

【 授权许可】

Unknown