Rhizobacteria Isolated from Saline Soil Induce Systemic Tolerance in Wheat (Triticum Aestivum L.) against Salinity Stress

Agronomy

Humaira Yasmin¹  Wajiha Khan²  Sumera Iqbal³  Noshin Ilyas⁴  Roomina Mazhar⁴  Hesham El Enshasy⁵  Daniel Joe Dailin⁵ 

[1] Department of Bio-Sciences, COMSATS University, Islamabad 45550, Pakistan;Department of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22010, Pakistan;Department of Botany, Lahore College for Women University, Lahore 54000, Pakistan;Department of Botany, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan;Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor 81310, Malaysia;

关键词: salinity;    PGPR;    wheat;    compatible solutes;    antioxidant enzymes;   

DOI  :  10.3390/agronomy10070989

来源: DOAJ

【 摘 要 】

Halo-tolerant plant growth-promoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agro-systems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR₁. SR₂, SR₃, and SR₄) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortium-inoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growth-promoting bacteria, isolated from salt-affected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions.

【 授权许可】

Unknown