Advances in Legionella Control by a New Formulation of Hydrogen Peroxide and Silver Salts in a Hospital Hot Water Network

Pathogens

Paolo Somaroli¹  Andrea Costa¹  Luna Girolamini²  Ada Dormi³  Francesca Savelli⁴  Andrea Martelli⁴  Davide Montanari⁵  Tiziana Pellati⁵  Sandra Cristino⁶  Antonella Grottola⁷  Giulia Fregni Serpini⁷ 

[1] Research, Lugo di Ravenna, 48022 Ravenna, Italy;Department of Biological, Geological, and Environmental Sciences, BiGeA, University of Bologna, 40126 Bologna, Italy;Department of Medical and Surgical Science, DIMEC, University of Bologna, 40126 Bologna, Italy;Eta-Beta S.r.l., 47122 Forlì, Forlì Cesena, Italy;;GVM Care &Regional Reference Laboratory for Clinical Diagnosis of Legionellosis, Unit of Microbiology and Virology, Modena University Hospital, 41125 Modena, Italy;Water Team S.r.l., 47522 Cesena, Forlì Cesena, Italy;

关键词: wtp 828;    legionella;    risk assessment plan;    water quality;    microbial analysis;    chemical analysis;   

DOI  :  10.3390/pathogens8040209

来源: DOAJ

【 摘 要 】

Legionella surveillance is an important issue in public health, linked to the severity of disease and the difficulty associated with eradicating this bacterium from the water environment. Different treatments are suggested to reduce Legionella risk, however long-term studies of their efficiency are lacking. This study focused on the activity of a new formulation of hydrogen peroxide and silver salts, WTP828, in the hospital hot water network (HWN) to contain Legionella contamination during two years of treatment. The effectiveness of WTP828 was tested measuring physical-chemical and microbiological parameters such as Legionella, Pseudomonas aeruginosa (P. aeruginosa), and a heterotopic plate count (HPC) at 36 °C. Legionella isolates were identified by serotyping and genotyping. WTP 828 induced a reduction in Legionella−positive sites (60% to 36%) and contamination levels (2.12 to 1.7 log₁₀ CFU/L), with isolates belonging to L. pneumophila SG1 (ST1 and ST104), L. anisa and L. rubrilucens widely distributed in HWN. No relevant contamination was found for other parameters tested. The long-term effect of WTP828 on Legionella containment suggest the easy and safe application of this disinfectant, that combined with knowledge of building characteristics, an adequate environmental monitoring and risk assessment plan, become the key elements in preventing Legionella contamination and exposure.

【 授权许可】

Unknown