Drosophila TRPγ is required in neuroendocrine cells for post-ingestive food selection

eLife

Youngseok Lee¹  Subash Dhakal¹  Qiuting Ren²  Craig Montell³  Jiangqu Liu³  Izel Tekin³  Bradley Akitake³ 

[1] Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of Korea;Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, United States;Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, United States;

关键词: feeding;    internal state;    metabolic sensor;    neuroendocrine cells;    TRP channel;    brain;   

DOI  :  10.7554/eLife.56726

来源: DOAJ

【 摘 要 】

The mechanism through which the brain senses the metabolic state, enabling an animal to regulate food consumption, and discriminate between nutritional and non-nutritional foods is a fundamental question. Flies choose the sweeter non-nutritive sugar, L-glucose, over the nutritive D-glucose if they are not starved. However, under starvation conditions, they switch their preference to D-glucose, and this occurs independent of peripheral taste neurons. Here, we found that eliminating the TRPγ channel impairs the ability of starved flies to choose D-glucose. This food selection depends on trpγ expression in neurosecretory cells in the brain that express diuretic hormone 44 (DH44). Loss of trpγ increases feeding, alters the physiology of the crop, which is the fly stomach equivalent, and decreases intracellular sugars and glycogen levels. Moreover, survival of starved trpγ flies is reduced. Expression of trpγ in DH44 neurons reverses these deficits. These results highlight roles for TRPγ in coordinating feeding with the metabolic state through expression in DH44 neuroendocrine cells.

【 授权许可】

Unknown