The Role of Chrysoperla carnea (Steph.) (Neuroptera: Chrysopidae) as a Potential Dispersive Agent of Noctuid Baculoviruses

Insects

Pilar Medina¹  OscarGiovanni Gutiérrez-Cárdenas¹  Ángeles Adán¹  Agustín Garzón¹  Primitivo Caballero²  Inés Beperet³ 

[1] Crop Protection Unit, School of Agricultural, Food and Biosystems Engineering, Technical University of Madrid, Puerta de Hierro, 2, 28040 Madrid, Spain;Institute for Multidisciplinary Research in Applied Biology, Public University of Navarre, 31006 Pamplona, Navarra, Spain;Research & Development Department, Bioinsectis SL. Pol. Ind. Mocholi Plaza Cein 5, Nave A14, 31110 Noain, Navarra, Spain;

关键词: AcMNPV;    excretion products;    horizontal transmission;    occlusion bodies;    SeMNPV;   

DOI  :  10.3390/insects11110760

来源: DOAJ

【 摘 要 】

Baculoviruses (BV) are highly effective against lepidopteran pests of economic importance such as Spodoptera exigua. The combined use of entomopathogens and macrobiological control agents requires the study of their relationships. Laboratory bioassays were developed to evaluate the interactions between the multiple nucleopolyhedroviruses of S. exigua (SeMNPV) and Autographa californica (AcMNPV), and the predator Chrysoperla carnea. The microscopic examination of predator’s excreta (larval drops and meconia) after the ingestion of BV-infected S. exigua revealed the presence of viral occlusion bodies (OBs). The reinfection of S. exigua larvae with BVs-contaminated excreta by using OBs water suspensions or by direct application both yielded high mortality values but different speed-of-kill results. Meconia killed before in suspensions due to their higher viral load and larval excretion drops did so in direct application due to their liquid nature and their easiness of consumption. The prey-mediated ingestion of SeMNPV and AcMNPV triggered slight effects in C. carnea, which were probably derived from the food nutritional quality. Chrysoperla carnea larvae did not discriminate between healthy and BV-infected S. exigua, while a preference was shown for S. exigua (healthy or infected) vs. Macrosiphum euphorbiae. Our findings present C. carnea, and particularly its larvae, as a promissory candidate for BV dispersion in the field.

【 授权许可】

Unknown