Recent Advances in Experimental Whole Genome Haplotyping Methods

International Journal of Molecular Sciences

Zuhong Lu¹  Mengting Huang¹  Jing Tu¹ 

[1] State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

关键词: haplotype;    haplotyping;    phasing;    next generation sequencing;   

DOI  :  10.3390/ijms18091944

来源: DOAJ

【 摘 要 】

Haplotype plays a vital role in diverse fields; however, the sequencing technologies cannot resolve haplotype directly. Pioneers demonstrated several approaches to resolve haplotype in the early years, which was extensively reviewed. Since then, numerous methods have been developed recently that have significantly improved phasing performance. Here, we review experimental methods that have emerged mainly over the past five years, and categorize them into five classes according to their maximum scale of contiguity: (i) encapsulation, (ii) 3D structure capture and construction, (iii) compartmentalization, (iv) fluorography, (v) long-read sequencing. Several subsections of certain methods are attached to each class as instances. We also discuss the relative advantages and disadvantages of different classes and make comparisons among representative methods of each class.

【 授权许可】

Unknown