Characterization of the Exo-Metabolome of the Emergent Phytopathogen Fusarium kuroshium sp. nov., a Causal Agent of Fusarium Dieback

Toxins

ClementeJ. García-Ávila¹  JoseAbel López-Buenfil²  Javier Plasencia³  Eliel Ruiz-May⁴  JuanL. Monribot-Villanueva⁵  JoséA. Guerrero-Analco⁵  Diana Sánchez-Rangel⁶  JoséB. Rodríguez-Haas⁶  Angélica Gutiérrez-Sánchez⁶ 

[1] Centro Nacional de Referencia Fitosanitaria del Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria, Tecámac, Estado de México 55740, Mexico;Colegio de Postgraduados, Carretera México-Texcoco Km 36.5 Texcoco, Estado de México 56101, Mexico;Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;Laboratorio de Proteómica, Red de Estudios Moleculares Avanzados, Clúster BioMimic^®, Instituto de Ecología, A. C. Xalapa, Veracruz 91073, Mexico;Laboratorio de Química de Productos Naturales, Red de Estudios Moleculares Avanzados, Clúster BioMimic^®, Instiuto de Ecología, A. C. Xalapa, Veracruz 91073, Mexico;Laboratorios de Fitopatología y Biología Molecular, Red de Estudios Moleculares Avanzados, Clúster BioMimic^®, Instituto de Ecología, A. C. Xalapa, Veracruz 91073, Mexico;

关键词: exo-metabolome;    fusaric acid;    Fusarium dieback;    Fusarium kuroshium;    fungal metabolomics;    Persea americana (avocado);   

DOI  :  10.3390/toxins13040268

来源: DOAJ

【 摘 要 】

Fusarium kuroshium is the fungal symbiont associated with the ambrosia beetle Euwallacea kuroshio, a plague complex that attacks avocado, among other hosts, causing a disease named Fusarium dieback (FD). However, the contribution of F. kuroshium to the establishment of this disease remains unknown. To advance the understanding of F. kuroshium pathogenicity, we profiled its exo-metabolome through metabolomics tools based on accurate mass spectrometry. We found that F. kuroshium can produce several key metabolites with phytotoxicity properties and other compounds with unknown functions. Among the metabolites identified in the fungal exo-metabolome, fusaric acid (FA) was further studied due to its phytotoxicity and relevance as a virulence factor. We tested both FA and organic extracts from F. kuroshium at various dilutions in avocado foliar tissue and found that they caused necrosis and chlorosis, resembling symptoms similar to those observed in FD. This study reports for first-time insights regarding F. kuroshium associated with its virulence, which could lead to the potential development of diagnostic and management tools of FD disease and provides a basis for understanding the interaction of F. kuroshium with its host plants.

【 授权许可】

Unknown