Expression of Calcium-Binding Proteins S100A2, S100A4 in Barrett's Adenocarcinomas

Neoplasia: An International Journal for Oncology Research

Mark Smoklin¹  Christopher A. Moskaluk²  Seung-Mo Hong³  Dunfa Peng⁴  Ok-Jae Lee⁴  Mohammad H. Razvi⁴  Wael El-Rifai⁴  Steven M. Powell⁵ 

[1] Department of Biostatistics, University of Virginia Health System, Charlottesville, VA, USA;Department of Internal Medicine, Institute of Health Science, College of Medicine, Gyeongsang National University, Jinju, Republic of Korea;Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA;Department of Surgery, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, USA;Division of Gastroenterology, University of Virginia Health System, Charlottesville, VA, USA;

关键词: S100A proteins;    Barrett's;    stomach;    adenocarcinomas;    overexpression;   

DOI  :  10.1593/neo.06481

来源: DOAJ

【 摘 要 】

In this study, we investigated the mRNA, protein expression of S100A2, S100A4 in adenocarcinomas of the stomach, esophagus. Real-time reverse transcription-polymerase reaction analysis on 72 tumors revealed frequent overexpression of S100A2, S100A4 in Barrett's adenocarcinomas (BAs) (P < .01). Immunohistochemical analysis on tumor tissue microarrays that contained 187 tumors showed absent to weak staining for S100A2 in all normal gastric mucosa samples, whereas normal esophageal mucosa samples demonstrated moderate to strong nuclear staining. Contrary to the nuclear expression of S100A2 in normal esophageal mucosa, two thirds of Barrett's dysplasia, BAs that overexpressed S100A2 demonstrated stronger cytosolic staining than nuclear staining (P < .001). Overexpression of S100A2 protein was more frequently seen in well-differentiated tumors than in others (P = .02). Moderate to strong staining of S100A4 was detected in two thirds of tumors, was frequently observed in the presence of Barrett's esophagus (P = .02). Similar to S100A2, the expression of S100A4 was predominantly cytosolic in two thirds of the tumors (P = .001). There was a significant correlation between S100A4 overexpression, lymph node metastasis (N2-N4) (P = .027). These results demonstrate frequent cytosolic overexpression of S100A2, S100A4 in BAs. Further studies are ongoing to underst, the biological significance of these S100A proteins in Barrett's tumorigenesis.

【 授权许可】

Unknown