Arbuscular Mycorrhizal Symbiosis Mitigates Iron (Fe)-Deficiency Retardation in Alfalfa (Medicago sativa L.) Through the Enhancement of Fe Accumulation and Sulfur-Assisted Antioxidant Defense

International Journal of Molecular Sciences

Ki-Won Lee¹  Md.Atikur Rahman¹  Sang-Hoon Lee¹  Monika Parvin²  EsratJahan Ela²  AhmadHumayan Kabir²  Urmi Das² 

[1] Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea;Molecular Plant Physiology Laboratory, Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh;

关键词: arbuscular mycorrhizal fungi;    symbiosis;    fe-deficiency;    alfalfa;    medicago sativa;    fe-mobilization;    fe-chelate reductase;   

DOI  :  10.3390/ijms21062219

来源: DOAJ

【 摘 要 】

Iron (Fe)-deficiency is one of the major constraints affecting growth, yield and nutritional quality in plants. This study was performed to elucidate how arbuscular mycorrhizal fungi (AMF) alleviate Fe-deficiency retardation in alfalfa (Medicago sativa L.). AMF supplementation improved plant biomass, chlorophyll score, Fv/Fm (quantum efficiency of photosystem II), and Pi_ABS (photosynthesis performance index), and reduced cell death, electrolyte leakage, and hydrogen peroxide accumulation in alfalfa. Moreover, AMF enhanced ferric chelate reductase activity as well as Fe, Zn, S and P in alfalfa under Fe-deficiency. Although Fe-transporters (MsIRT1 and MsNramp1) did not induce in root but MsFRO1 significantly induced by AMF under Fe deficiency in roots, suggesting that AMF-mediated Fe enhancement is related to the bioavailability of Fe at rhizosphere/root apoplast rather than the upregulation of Fe transporters under Fe deficiency in alfalfa. Several S-transporters (MsSULTR1;1, MsSULTR1;2, MsSULTR1;3, and MsSULTR3;1) markedly increased following AMF supplementation with or without Fe-deficiency alfalfa. Our study further suggests that Fe uptake system is independently influenced by AMF regardless of the S status in alfalfa. However, the increase of S in alfalfa is correlated with the elevation of GR and S-metabolites (glutathione and cysteine) associated with antioxidant defense under Fe deficiency.

【 授权许可】

Unknown