Comparative Analysis of Proteomics and Transcriptomics during Fertility Transition in a Two-Line Hybrid Rice Line Wuxiang S

International Journal of Molecular Sciences

Baojin Zhou¹  Ruo Zhou¹  Hongyuan Zhang²  Jing Jin³  Yu Zou³  Yi Ding³  Qian Li³  Hao Chen³  Ying Wang³  Xingguo Huang⁴ 

[1] Deepxomics Co., Ltd., Shenzhen 518000, China;Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan 430065, China;State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China;Wuhan Wuda Tianyuau Bio-Tech Co., Ltd., Wuhan 430070, China;

关键词: rice (Oryza sativa L.);    male sterile line;    iTRAQ;    RNA-seq;    differentially abundant proteins;   

DOI  :  10.3390/ijms20184542

来源: DOAJ

【 摘 要 】

The two-line hybrid rice is an important factor of a global crop, but its fertility transition mechanism is unclear. Here, a comparative proteomics and transcriptomics analysis was completed on the two-line hybrid rice line Wuxiang S (WXS) to explore its molecular mechanism and protein regulation during fertility transition. A total of 340 differentially abundant proteins (DAPs) were identified using iTRAQ between the pollen mother cell formation stage (P2) and the meiosis stage (P3). There were 3541 and 4247 differentially expressed genes (DEGs) in P2 and P3 between WXS (Sterile, S)-WXS(S) and WXS (Fertile, F)-WXS(F), respectively, of which 92 and 71 DEGs had corresponding DAPs. Among the DAPs and DEGs, 65 (SP2 vs. FP2) and 55 (SP3 vs. FP3) corresponding DEGs and DAPs (cor-DEGs-DAPs) showed the same expression trend, indicating the cor-DEGs-DAPs genes might play vital roles in WXS fertility transition. Further analysis indicated that cor-DEGs-DAPs proteins were related to energy metabolism-related proteins in anther development and were accompanied by the activation of the stress response pathway and modifications to the cell wall, which ultimately affected the fertility transition of the PTGMS rice line WXS.

【 授权许可】

Unknown