Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses

International Journal of Environmental Research and Public Health

Megan Opichka¹  MarkL. McGlynn²  Dustin Slivka²  ChristopherW. Collins²  Camille Larson² 

[1] Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA;School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA;

关键词: RT-qPCR;    transcription;    truncated;    alternate promoter;    primary promoter;   

DOI  :  10.3390/ijerph17165740

来源: DOAJ

【 摘 要 】

Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response.

【 授权许可】

Unknown