Screening transesterifiable lipid accumulating bacteria from sewage sludge for biodiesel production

Biotechnology Reports

Naiane Sangaletti-Gerhard¹  Francisco Osses²  Mara Cea²  Pedro Acuña²  Idi Fuentes²  Karina Godoy²  Rodrigo Navia²  Milko Jorquera² 

[1] Laboratory of Oils and Fats, Department of Agro-food Industry, Food and Nutrition, College of Agriculture “Luiz de Queiroz” (ESALQ), University of São Paulo, Piracicaba, São Paulo, Brazil;Scientific and Technological Bioresource Nucleus, University of La Frontera, P.O. Box 54-D, Temuco, Chile;

关键词: Activated sludge;    Bacillus sp.;    Biodiesel;    Lipids;    Fatty acid methyl esters;   

DOI  :  10.1016/j.btre.2015.10.008

来源: DOAJ

【 摘 要 】

Sewage sludge was evaluated as high available and low cost microbial oils feedstock for biodiesel production. Samples from four different wastewater treatment plants from La Araucanía Region in Southern Chile presented total lipids content ranging between 7.7 and 12.6%, being Vilcún sewage sludge that with the highest transesterifiable lipids content of about 50% of the total extracted lipids. The most relevant identified bacteria present in sludge samples were Acinetobacter, Pseudomonas and Bacillus, being Bacillus sp. V10 the strain with the highest transesterfiable lipids content of 7.4%. Bacillus sp. V10 was cultured using urban wastewater supplemented with glucose to achieve nitrogen depleted medium and using milk processing wastewater as a low-cost carbon source. Bacillus sp. V10 lipid profile indicates that low degree unsaturated long chain fatty acids such as C18:1 may account for approximately 50% of the lipids content, indicating its suitability to be used as raw material for biodiesel production.

【 授权许可】

Unknown