IL-17 Facilitates VCAM-1 Production and Monocyte Adhesion in Osteoarthritis Synovial Fibroblasts by Suppressing miR-5701 Synthesis

International Journal of Molecular Sciences

Chao-Yang Lai¹  Chih-Yuan Ko²  Tsung-Ju Wu³  Chun-Hao Tsai⁴  Yi-Chin Fong⁴  Chen-Ming Su⁴  Chih-Hsin Tang⁵  Sunny Li-Yun Chang⁵  Xiu-Yuan He⁵  Chih-Yang Lin⁶ 

[1] Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung 413305, Taiwan;Department of Orthopedic Surgery, China Medical University Hospital, Taichung 404327, Taiwan;Department of Physical Medicine and Rehabilitation, Changhua Christian Hospital, Changhua 500209, Taiwan;Department of Sports Medicine, College of Health Care, China Medical University, Taichung 406040, Taiwan;Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, Taiwan;Translational Medicine Center, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei 111045, Taiwan;

关键词: IL-17;    osteoarthritis;    monocytes;    VCAM-1;    adhesion;   

DOI  :  10.3390/ijms23126804

来源: DOAJ

【 摘 要 】

Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocytes into the inflamed joint synovium. Interleukin (IL)-17 is a critical inflammatory mediator that participates in the progression of OA, although the mechanisms linking IL-17 and monocyte infiltration are not well understood. Our analysis of synovial tissue samples retrieved from the Gene Expression Omnibus (GEO) dataset exhibited higher monocyte marker (CD11b) and vascular cell adhesion molecule 1 (VCAM-1) levels in OA samples than in normal, healthy samples. The stimulation of human OA synovial fibroblasts (OASFs) with IL-17 increased VCAM-1 production and subsequently enhanced monocyte adhesion. IL-17 affected VCAM-1-dependent monocyte adhesion by reducing miR-5701 expression through the protein kinase C (PKC)-α and c-Jun N-terminal kinase (JNK) signaling cascades. Our findings improve our understanding about the effect of IL-17 on OA progression and, in particular, VCAM-1 production and monocyte adhesion, which may help with the design of more effective OA treatments.

【 授权许可】

Unknown