Workflow for the Quantification of Soluble and Insoluble Carbohydrates in Soybean Seed

Molecules

AnaP. Alonso¹  Ademar Moretti¹  CintiaL. Arias¹  LeandroA. Mozzoni²  LeahK. McHale³  Pengyin Chen⁴  BrantT. McNeece⁵  M.A.Rouf Mian⁵ 

[1] BioDiscovery Institute, University of North Texas, Denton, TX 76201, USA;Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA;Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH 43210, USA;Fisher Delta Research Center, University of Missouri, Portageville, MO 63873, USA;USDA-ARS, Soybean & Nitrogen Fixation Unit, Raleigh, NC 27607, USA;

关键词: carbohydrates;    soybean;    oil;    protein;    soluble sugars;    starch;   

DOI  :  10.3390/molecules25173806

来源: DOAJ

【 摘 要 】

Soybean seed composition has a profound impact on its market value and commercial use as an important commodity. Increases in oil and protein content have been historically pursued by breeders and genetic engineers; consequently, rapid methods for their quantification are well established. The interest in complete carbohydrate profiles in mature seeds, on the other hand, has recently increased due to numerous attempts to redirect carbohydrates into oil and protein or to offer specialty seed with a specific sugar profile to meet animal nutritional requirements. In this work, a sequential protocol for quantifying reserve and structural carbohydrates in soybean seed was developed and validated. Through this procedure, the concentrations of soluble sugars, sugar alcohols, starch, hemicellulose, and crystalline cellulose can be determined in successive steps from the same starting material using colorimetric assays, LC–MS/MS, and GC–MS. The entire workflow was evaluated using internal standards to estimate the recovery efficiency. Finally, it was successfully applied to eight soybean genotypes harvested from two locations, and the resulting correlations of carbohydrate and oil or protein are presented. This methodology has the potential not only to guide soybean cultivar optimization processes but also to be expanded to other crops with only slight modifications.

【 授权许可】

Unknown