Feasibility Study for Detection of Turnip yellow mosaic virus (TYMV) Infection of Chinese Cabbage Plants Using Raman Spectroscopy

The Plant Pathology Journal

Mi-Kyeong Kim¹  Jeong-Soo Kim¹  Saetbyeol Kim²  Sanguk Lee²  Hoeil Chung²  Su-Heon Lee³  Hee-Youn Chi⁴ 

[1] Agricultural Microbiology Division, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea;Department of Chemistry, Hanyang University, Seoul 133-791, Korea;School of Applied Biosciences, College of Agriculture & Life Scieneces, Kyungpook National University, Daegu 702-701, Korea;Smarteome. Co., Ltd., Suwon 441-853, Korea;

关键词: discrimination;    Raman spectroscopy;    surface enhanced Raman scattering (SERS);    Turnip yellow mosaic virus (TYMV);    virus detection;   

DOI  :  10.5423/PPJ.NT.09.2012.0147

来源: DOAJ

【 摘 要 】

Raman spectroscopy provides many advantages compared to other common analytical techniques due to its ability of rapid and accurate identification of unknown specimens as well as simple sample preparation. Here, we described potential of Raman spectroscopic technique as an efficient and high throughput method to detect plants infected by economically important viruses. To enhance the detection sensitivity of Raman measurement, surface enhanced Raman scattering (SERS) was employed. Spectra of extracts from healthy and Turnip yellow mosaic virus (TYMV) infected Chinese cabbage leaves were collected by mixing with gold (Au) nanoparticles. Our result showed that TYMV infected plants could be discriminated from non-infected healthy plants, suggesting the current method described here would be an alternative potential tool to screen virus-infection of plants in fields although it needs more studies to generalize the technique.

【 授权许可】

Unknown