Leucocyte-Rich Platelet-Rich Plasma Enhances Fibroblast and Extracellular Matrix Activity: Implications in Wound Healing

International Journal of Molecular Sciences

Jeannie Devereaux¹  Dimitrios Kiatos¹  Sarah Fraser²  Kulmira Nurgali²  Vasso Apostolopoulos²  Narges Dargahi² 

[1] College of Health and Biomedicine, Victoria University, Melbourne, VIC 3011, Australia;Institute for Health and Sport, Victoria University, Melbourne, VIC 3011, Australia;

关键词: platelet-rich plasma;    PRP;    platelet gel;    platelet-rich fibrin;    fibroblasts;    extracellular matrix;   

DOI  :  10.3390/ijms21186519

来源: DOAJ

【 摘 要 】

Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. L iterature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 10³ platelets/µL and 16.36 ± 2.08 × 10³ white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 10³ platelets/µL and 0.8 ± 0.02 × 10³ white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.

【 授权许可】

Unknown