Comprehensive Molecular Dissection of Dermatophilus congolensis Genome and First Observation of tet(Z) Tetracycline Resistance

International Journal of Molecular Sciences

Filip Boyen¹  Matylda Barbara Mielcarska²  Lidia Szulc-Dąbrowska²  Shevaun Johnson³  Aspinas Chapwanya³  Patrick Butaye³  Ian Branford³  Felix Ngosa Toka³  Samantha Zayas³ 

[1] Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium;Institute of Veterinary Medicine, Warsaw University of Life Sciences—SGGW, 02-786 Warsaw, Poland;Ross University School of Veterinary Medicine, 42123 Basseterre, Saint Kitts and Nevis;

关键词: Dermatophilus congolensis;    genome;    tet(Z);    antimicrobial resistance AMR;    virulence factors;    secondary metabolites;   

DOI  :  10.3390/ijms22137128

来源: DOAJ

【 摘 要 】

Dermatophilus congolensis is a bacterial pathogen mostly of ruminant livestock in the tropics/subtropics and certain temperate climate areas. It causes dermatophilosis, a skin disease that threatens food security by lowering animal productivity and compromising animal health and welfare. Since it is a prevalent infection in ruminants, dermatophilosis warrants more research. There is limited understanding of its pathogenicity, and as such, there is no registered vaccine against D. congolensis. To better understanding the genomics of D. congolensis, the primary aim of this work was to investigate this bacterium using whole-genome sequencing and bioinformatic analysis. D. congolensis is a high GC member of the Actinobacteria and encodes approximately 2527 genes. It has an open pan-genome, contains many potential virulence factors, secondary metabolites and encodes at least 23 housekeeping genes associated with antimicrobial susceptibility mechanisms and some isolates have an acquired antimicrobial resistance gene. Our isolates contain a single CRISPR array Cas type IE with classical 8 Cas genes. Although the isolates originate from the same geographical location there is some genomic diversity among them. In conclusion, we present the first detailed genomic study on D. congolensis, including the first observation of tet(Z), a tetracycline resistance-conferring gene.

【 授权许可】

Unknown