Antibiofilm activity of silver nanoparticles against biofilm forming Staphylococcus pseudintermedius isolated from dogs with otitis externa

Veterinary Medicine and Science

Mimi Seo¹  Seulgi Bae¹  Taeho Oh¹ 

[1] Department of Veterinary Internal Medicine College of Veterinary Medicine Kyungpook National University Daegu Republic of Korea;

关键词: antibiofilm;    dog;    otitis externa;    silver nanoparticles;    Staphylococcus pseudintermedius;   

DOI  :  10.1002/vms3.554

来源: DOAJ

【 摘 要 】

Background Silver nanoparticles (AgNPs) are known to possess antimicrobial properties. Although the antibiofilm activity of AgNPs has been demonstrated in humans, this activity has not yet been elucidated in veterinary medicine. Objectives The purpose of this study was to evaluate the antibiofilm activity of silver nanoparticles against Staphylococcus pseudintermedius. Methods Ten isolates of S. pseudintermedius obtained from dogs with otitis externa were treated with AgNPs, and the antibiofilm activity was measured using a modified microtiter plate and Congo red agar (CRA) method and scanning electron microscopy. Results AgNPs displayed a significant dose‐dependent antibiofilm activity and reduced biofilm formation at concentrations of 20 and 10 µg/ml (p < 0.05). S. pseudintermedius exposed to 20 µg/ml of AgNPs formed less bacterial slime compared to the controls on CRA plates. Scanning electron micrographs showed that the biofilm had few individually scattered cells along its surface when treated with AgNP concentrations of 20 and 10 µg/ml. Untreated surfaces showed an aggregated biofilm. Conclusions Our results suggested that AgNP may be a valuable alternative antibiofilm agent for canine otitis externa.

【 授权许可】

Unknown