Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice

eLife

Hanbing Song¹  Xueying Wang²  John A Hayes²  Gregory D Funk²  Andrew Kottick²  Victoria T Akins²  Nikolas C Vann²  M Drew LaMar²  Maria Cristina D Picardo³  Ann L Revill⁴  Christopher A Del Negro⁴ 

[1] The Women and Children's Health Research Institute, University of Alberta, Edmonton, Canada;Department of Applied Science, The College of William and Mary, Williamsburg, United States;Department of Biology, The College of William and Mary, Williamsburg, United States;Department of Physiology, University of Alberta, Edmonton, Canada;

关键词: respiration;    breathing;    central pattern generator;    two-photon microscopy;    preBötzinger complex;   

DOI  :  10.7554/eLife.03427

来源: DOAJ

【 摘 要 】

To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm generator in mammals are derived from Dbx1-expressing precursors. Here, we show that selective photonic destruction of Dbx1 preBötC neurons in neonatal mouse slices impairs respiratory rhythm but surprisingly also the magnitude of motor output; respiratory hypoglossal nerve discharge decreased and its frequency steadily diminished until rhythm stopped irreversibly after 85±20 (mean ± SEM) cellular ablations, which corresponds to ∼15% of the estimated population. These results demonstrate that a single canonical interneuron class generates respiratory rhythm and contributes in a premotor capacity, whereas these functions are normally attributed to discrete populations. We also establish quantitative cellular parameters that govern network viability, which may have ramifications for respiratory pathology in disease states.

【 授权许可】

Unknown