Upregulation of miR-133b and miR-328 in Patients With Atrial Dilatation: Implications for Stretch-Induced Atrial Fibrillation

Frontiers in Physiology

Margherita Grasso¹  Manuel Nicolussi Giacomaz¹  Michela Alessandra Denti¹  Laura Avogaro¹  Angelo Graffigna²  Francesco Tessarolo³  Michela Masè³  Flavia Ravelli⁴  Elvira D’Amato⁴ 

[1] Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy;Division of Cardiac Surgery, Santa Chiara Hospital, Trento, Italy;Healthcare Research and Innovation Program, Bruno Kessler Foundation, Trento, Italy;Laboratory of Biophysics and Biosignals, University of Trento, Trento, Italy;

关键词: chronic stretch;    microRNA;    atrial fibrillation;    arrhythmic substrate;    post-transcriptional regulation;    atrial remodeling;   

DOI  :  10.3389/fphys.2019.01133

来源: DOAJ

【 摘 要 】

Atrial stretch and dilatation are common features of many clinical conditions predisposing to atrial fibrillation (AF). MicroRNAs (miRs) are emerging as potential molecular determinants of AF, but their relationship with atrial dilatation (AD) is poorly understood. The present study was designed to assess the specific miR expression profiles associated with AD in human atrial tissue. The expressions of a preselected panel of miRs, previously described as playing a role in cardiac disease, were quantified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in atrial tissue samples from 30 cardiac surgery patients, who were characterized by different grades of AD and arrhythmic profiles. Our results showed that AD per se was associated with significant up-regulation of miR-328-3p and miR-133b (p < 0.05) with respect to controls, with a fold-change of 1.53 and 1.74, respectively. In a multivariate model including AD and AF as independent variables, miR-328-3p expression was mainly associated with AD grade (p < 0.05), while miR-133b was related to both AD (p < 0.005) and AF (p < 0.05), the two factors exerting opposite modulation effects. The presence of AF was associated with significant (p < 0.05) up-regulation of the expression level of miR-1-3p, miR-21-5p, miR-29a-3p, miR-208b-3p, and miR-590-5p. These results showed the existence of specific alterations of miR expression associated with AD, which may pave the way to future experimental studies to test the involvement of post-transcriptional mechanisms in the stretch-induced formation of a pro-arrhythmic substrate.

【 授权许可】

Unknown