Biofabrication of Cell-Laden Gelatin Methacryloyl Hydrogels with Incorporation of Silanized Hydroxyapatite by Visible Light Projection

Polymers

Hsuan Chen¹  Shyh-Yuan Lee¹  Yuan-Min Lin¹  Chih-Hsin Lin²  Jimmy Jiun-Ming Su³ 

[1] Department of Dentistry, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan;Graduate Institute of Nanomedicine and Medical Engineering, Taipei Medical University, Taipei 110, Taiwan;Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University, Taipei 112, Taiwan;

关键词: gelatin methacrylate;    hydroxyapatite;    silanization;    mesenchymal stem cells;    digital light processing projection;    3D printing;   

DOI  :  10.3390/polym13142354

来源: DOAJ

【 摘 要 】

Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA hydrogel is its poor mechanical properties, which may compromise the feasibility of biofabrication techniques. In this study, a cell-laden GelMA composite hydrogel with a combination incorporating silanized hydroxyapatite (Si-HAp) and a simple and harmless visible light crosslinking system for this hydrogel were developed. The incorporation of Si-HAp into the GelMA hydrogel enhanced the mechanical properties of the composite hydrogel. Moreover, the composite hydrogel exhibited low cytotoxicity and promoted the osteogenic gene expression of embedded MG63 cells and Human bone marrow mesenchymal stem cells (hBMSCs). We also established a maskless lithographic method to fabricate a defined 3D structure under visible light by using a digital light processing projector, and the incorporation of Si-HAp increased the resolution of photolithographic hydrogels. The GelMA-Si-HAp composite hydrogel system can serve as an effective biomaterial in bone regeneration.

【 授权许可】

Unknown