Mucosal Microbiome Profiles Polygenic Irritable Bowel Syndrome in Mestizo Individuals

Frontiers in Cellular and Infection Microbiology

Consorcio Mexicano para el Estudio del Microbioma Humano¹  Miguel A. Cevallos²  Luis David Alcaraz³  Angel-Mario Zarate⁴  Max Schmulson⁵  Rene Arredondo-Hernández⁶  Samuel Ponce-de-León Rosales⁶  Antonio R. Villa⁶  Patricia Orduña⁶  Gamaliel López-Leal⁷  Yolanda López-Vidal⁷  Rubí Santiago-Cruz⁷  Gerardo Alanis-Funes⁸ 

[1] ;Centro de Ciencias Genómicas, Programa de Genómica Evolutiva, Universidad Nacional Autónoma de México, Cuernavaca, Mexico;Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico;Hospital General de México, Dr. Eduardo Liceaga, Mexico City, Mexico;Laboratorio de Hígado, Páncreas y Motilidad (HIPAM), Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico;Laboratorio de Microbioma, División de Estudios de Posgrado y División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico;Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico;Tecnologico de Monterrey, School of Medicine and Health Sciences, Monterrey, Mexico;

关键词: IBS;    SNPs;    microbiota;    Bacteroides;    polygenic SNPs;   

DOI  :  10.3389/fcimb.2020.00072

来源: DOAJ

【 摘 要 】

Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunction with colonic mucosa dysbiosis, is necessary to determine IBS physiopathology and the effects of environmental pressures. Using a simple polygenic model, host single nucleotide polymorphisms (SNPs) and the taxonomic compositions of microbiota were compared between IBS patients and healthy subjects. As proof of concept, five IBS-Rome III patients and five healthy controls (HCs) were systematically studied. The human and bacterial intestinal metagenome of each subject was taxonomically annotated and screened for previously annotated IBS, ulcerative colitis, and Crohn's disease-associated SNPs or taxon abundance. Dietary data and fecal markers were collected and associated with the intestinal microbiome. However, more than 1,000 variants were found, and at least 76 SNPs differentiated IBS patients from HCs, as did associations with 4 phyla and 10 bacterial genera. In this study, we found elements supporting a polygenic background, with frequent variants, among the Mestizo population, and the colonic mucosal enrichment of Bacteroides, Alteromonas, Neisseria, Streptococcus, and Microbacterium, may serve as a hallmark for IBS.

【 授权许可】

Unknown