Isolation, antioxidant property and protective effect on PC12 cell of the main anthocyanin in fruit of Lycium ruthenicum Murray

Journal of Functional Foods

Linwu Ran¹  Xiaoxiong Zeng²  Youlong Cao²  Yamei Yan²  Lu Lu²  Jilong Tang²  Jia Mi³  Yuxiao Gao⁴  Yi Sun⁴ 

[1] National Wolfberry Engineering Research Center, Yinchuan 750002, People’s Republic of China;College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China;Laboratory Animal Center, Ningxia Medical University, Yinchuan 750004, People’s Republic of China;National Wolfberry Engineering Research Center, Yinchuan 750002, People’s Republic of China;

关键词: Lycium ruthenicum;    Anthocyanin;    Antioxidant property;    PC12 cell;    Cytoprotective effect;   

DOI  :  

来源: DOAJ

【 摘 要 】

The fruit of Lycium ruthenicum Murray has been widely used as a precious Chinese herbal medicine. In this study, the major anthocyanin in L. ruthenicum fruit was obtained via an AKTA purifier system with YMC-Pack ODS-A column and identified by mass and nuclear magnetic resonance spectroscopy to be petunidin 3-O-[6-O-(4-O-(trans-p-coumaroyl)-α-l-rhamnopyranosyl)-β-d-glucopyranoside]-5-O-[β-d-glucopyranoside] (compound 1). Compound 1 exhibited higher scavenging activities against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 1,1-diphenyl-2-picrylhydra-zyl and superoxide radicals than the crude extract of anthocyanins. Moreover, compound 1 was demonstrated to have potential cytoprotective effect on hydrogen peroxide-induced intracellular reactive oxygen damage in neuronlike rat pheochromocytoma line cells by promoting cell proliferation, mitigating lipid peroxidation and regulating endogenous antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase).

【 授权许可】

Unknown